iκb kinase beta phosphorylation Search Results


p pi3k  (Bioss)
95
Bioss p pi3k
P Pi3k, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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casein kinase ii ck2  (New England Biolabs)
95
New England Biolabs casein kinase ii ck2
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Casein Kinase Ii Ck2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated p gsk 3β  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphorylated p gsk 3β
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Phosphorylated P Gsk 3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt protein kinase b pkb  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc akt protein kinase b pkb
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Akt Protein Kinase B Pkb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iκb kinase ikk β antibodies  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology iκb kinase ikk β antibodies
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Iκb Kinase Ikk β Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated glycogen synthase kinase 3β  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3β
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Phosphorylated Glycogen Synthase Kinase 3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p iκb kinase ikk α β  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p iκb kinase ikk α β
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
P Iκb Kinase Ikk α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tyr1162/tyr1163-phosphorylated insulin receptors antibody  (Millipore)
90
Millipore tyr1162/tyr1163-phosphorylated insulin receptors antibody
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Tyr1162/Tyr1163 Phosphorylated Insulin Receptors Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated glycogen synthase kinase 3β p gsk3β ser 9  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphorylated glycogen synthase kinase 3β p gsk3β ser 9
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Phosphorylated Glycogen Synthase Kinase 3β P Gsk3β Ser 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphor gsk3α β  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phosphor gsk3α β
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Phosphor Gsk3α β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro gsk 3β kinase phosphorylation assay recombinant purified mouse il 22r  (R&D Systems)
93
R&D Systems vitro gsk 3β kinase phosphorylation assay recombinant purified mouse il 22r
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Vitro Gsk 3β Kinase Phosphorylation Assay Recombinant Purified Mouse Il 22r, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti iκb kinase β ikkβ  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti iκb kinase β ikkβ
( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or <t>CK2</t> (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.
Anti Iκb Kinase β Ikkβ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or CK2 (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.

Journal: PLoS ONE

Article Title: Decreased Camptothecin Sensitivity of the Stem-Cell-Like Fraction of Caco2 Cells Correlates with an Altered Phosphorylation Pattern of Topoisomerase I

doi: 10.1371/journal.pone.0099628

Figure Lengend Snippet: ( A ) The TopI activity in CD44+ cell extracts after phosphorylation or dephosphorylation. Whole cell extract was either left untreated (indicated by Control) or incubated with phosphatase (indicated by Dephos) or CK2 (indicated by Phos) before TopI activity was measured by REEAD in the presence of 60 µM CPT or 5% DMSO as indicated in the figure. One example randomly picked out of 12 individual microscopic images of each reaction sample is shown in the top panel. A quantitative depiction of three independent experiments, obtained as described in the legend of is shown in the lower panel. ( B ) Same as ( A ), except that the analyses were performed on extracts from CD44− cells.

Article Snippet: CD44 MicroBeads (human), FcR Blocking Reagent (human), CD44-PE Antibody (human), CD133-PE Antibody (human), Casein Kinase II (CK2) and alkaline phosphatase from calf intestine were purchased from New England BioLabs Ltd. (UK).

Techniques: Activity Assay, De-Phosphorylation Assay, Incubation